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. 2020 Nov 10;9:e60939. doi: 10.7554/eLife.60939

Figure 2. Progenitor cell populations in the bone marrow compartment (A–G) and in the circulation (H and I).

Control individuals (white bars, n = 13) and individuals with CAD (gray bars, n = 13). HSC and MPP cell populations were combined as the CD90 expression marker was not available for n = 6 in each study group. Geometric mean with 95% CI. The p-values are corrected for age and BMI with ANCOVA. * indicates p<0.05, **: p<0.01. In top-down order: HSC indicates hematopoietic stem cell, MPP: multipotent progenitor, CLP: common lymphoid progenitor, CMP: common myeloid progenitor, GMP: granulocyte-macrophage progenitor, MEP: megakaryocyte erythrocyte progenitor.

Figure 2.

Figure 2—figure supplement 1. Gating strategy of hematopoietic stem and progenitor cells in the bone marrow.

Figure 2—figure supplement 1.

HSPCs were defined as CD45+CD34+CD38dim cells, after exclusion of dead cells and doublets. Next, the lymphoid lineage was excluded in CD19-CD117+ cells. In CD45RAdimCD38+ cells, CMP, GMP, MEP, and R1-3 progenitor populations were identified using CD123 and CD45RA expression, see Table for details. CD90 expression in CD38-CD45RA- cells determined MPP and HSC populations.