Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Homo sapiens) | Peripheral blood | Through venous puncture | Freshly isolated from Homo sapiens, men, 18–75 years | |
| Biological sample (Homo sapiens) | Bone Marrow aspirate | From the posterior iliac crest according to standard practice |
Freshly isolated from Homo sapiens | |
| Antibody | Mouse monoclonal CD45 KO | Beckman Coulter | Clone J33 Cat# B36294, RRID:AB_2833027 | (1:25) |
| Antibody | Mouse monoclonal HLA-DR PE | Beckman Coulter | Clone immu-357 Cat# IM1639U RRID:AB_2876782 | (1:10) |
| Antibody | Mouse monoclonal CD14 PECy7 | eBioscience | Clone 61D3 Cat# 25-0149-42 RRID:AB_1582276 |
(1:25) |
| Antibody | Mouse monoclonal CD16 FITC | eBioscience | Clone CB16 Cat# 11-0168-42 RRID:AB_10805747 |
(1:25) |
| Antibody | Mouse monoclonal CD3 APC-Alexa750 | Beckman Coulter | Clone UCTH1 Cat# A66329 RRID:AB_2876783 |
(1:25) |
| Antibody | Mouse monoclonal CD56 APC | Beckman Coulter | Clone N901 Cat# IM2474U RRID:AB_2876784 |
(1:25) |
| Antibody | Mouse monoclonal CD192 BV421 | BD Biosciences | Clone 48607 Cat# 564067, RRID:AB_2738573 | (1:50) |
| Antibody | Mouse monoclonal CD11b BV785 | Biolegend | Clone ICRF44 Cat# 301346, RRID:AB_2563794 |
(1:50) |
| Antibody | Mouse monoclonal CD41 PerCP-Cy5.5 | Biolegend | Clone Hip8 Cat# 303719, RRID:AB_2561731 |
(1:50) |
| Antibody | Mouse monoclonal CD90 FITC | Biolegend | Clone 5E10 Cat# 328107, RRID:AB_893438 | (1:50) |
| Antibody | Mouse monoclonal CD123 PE | BD Biosciences | Clone 9F5 Cat# 555644, RRID:AB_396001 | (1:40) |
| Antibody | Mouse monoclonal CD19 ECD | Beckman Coulter | Clone J4.119 Cat# IM2708U, RRID:AB_130854 | (1:20) |
| Antibody | Mouse monoclonal CD38 PC5.5 | Beckman Coulter | Clone LS198-4-3 Cat# IM2651U, RRID:AB_131166 | (1:20) |
| Antibody | Mouse monoclonal CD117 PEC7 | Beckman Coulter | Clone 104D2D1 Cat# IM3698, RRID:AB_131184 | (1:20) |
| Antibody | Mouse monoclonal CD45RA APC | Beckman Coulter | Clone 2H4LDH11LD89 (2H4) Cat# B14807 RRID:AB_2876787 |
(1:20) |
| Antibody | Mouse monoclonal CD34-APC A750 | Beckman Coulter | Clone 581 Cat# A89309 RRID:AB_2876786 |
(1:20) |
| Commercial assay or kit | DRAQ7 | Biostatus | Live/Dead stain | (1:500) |
| Commercial assay or kit | Human Cytokine Magnetic Magpix 25-plex panel | Invitrogen | MAGPIX platform | |
| Commercial assay or kit | SimplePlex cartridge | ProteinSimple | Ella platform | |
| Commercial assay or kit | Truseq small RNA primers | Illumina | ||
| Commercial assay or kit | hsCRP ELISA | R&D | DY1707 | |
| Commercial assay or kit | VCAM-1 ELISA | R&D | DY809 | |
| Commercial assay or kit | MMP2 ELISA | R&D | DY902 | |
| Commercial assay or kit | E-selectin ELISA | R&D | DY724 | |
| Chemical compound, drug | Pharm Lyse lysing buffer | BD Biosciences | ||
| Chemical compound, drug | Glutamine | Invitrogen | 2 mmol/L in RPMI | |
| Chemical compound, drug | Gentamycin | Centrafarm | 10 mg/mL in RPMI | |
| Chemical compound, drug | Pyruvate | Invitrogen | 1 mmol/L in RPMI | |
| Chemical compound, drug | Methocult GF | Stemcell Technologies | H84435 | |
| Sequence-based reagent | Hg19 human Refseq transcriptome | Li and Durbin, 2010 | To align RNAseq | |
| Peptide, recombinant protein | Lipopolysaccharide from Escherichia coli | Sigma-Aldrich | Serotype 055:B5, L2880 | 10 ng/mL |
| Peptide, recombinant protein | Pam3CysK4 | EMC Microcollections | L2000 | 10 ug/mL |
| Peptide, recombinant protein | Interferon gamma | Immukine, Boehringer Ingelheim BV | 50 ng/mL for Seahorse | |
| Peptide, recombinant protein | Oligomycin | Sigma-Aldrich | 75351 | 1 mM for Seahorse |
| Peptide, recombinant protein | FCCP | Sigma-Aldrich | C2920 | 1 mM for Seahorse |
| Peptide, recombinant protein | Rotenone | Sigma-Aldrich | R8875 | 1.25 mM |
| Peptide, recombinant protein | Antimycin A | Sigma-Aldrich | A8674 | 2.5 mM |
| Software, algorithm |
Kaluza | Beckman Coulter | Version 2.1 RRID:SCR_016182 | Flow Cytometry analysis |
| Software, algorithm | MultiQC | Ewels et al., 2016 | RRID:SCR_014982 | Quality check RNAseq |
| Software, algorithm | DEseq2 v1.22.0 |
Love et al., 2014
BioConductor |
RRID:SCR_015687 | Differential gene expression RNAseq |
| Software, algorithm | clusterProfiler v3.10.1 |
Yu et al., 2012
BioConductor |
RRID:SCR_016884 | RNAseq |
| Software, algorithm | R 3.6.1 | https://www.r-project.org/ | RRID:SCR_001905 | |
| Software, algorithm | TrueX algorithm | EARL protocols | ||
| Software, algorithm | Inveon Research Workspace 4.2 | Preclinical Solutions, Siemens Medical Solutions | 3D Gaussian filter kernel, 3.0 mm | Postprocessing of FDG PET CT scanning |
| Software, algorithm | PyRadiomics toolbox | van Griethuysen et al., 2017 | Analysis FDG PET CT | |
| Software, algorithm | SPSS V25.0 | SPSS | RRID:SCR_002865 | Data analysis |
| Software, algorithm | Prism v6.0 | GraphPad software | RRID:SCR_002798 | Figures |
| Other | Sysmex-XN 450 hematology analyzer | Sysmex | For total blood counts | |
| Other | CytoFLEX flow cytometer | Beckman Coulter | 13 color on CytExpert RRID:SCR_017217 | Flow Cytometry Peripheral blood |
| Other | Navios flow cytometer | Beckman Coulter | RRID:SCR_014421 | Flow Cytometry Bone marrow Progenitors |
| Other | XFp Analyzer | Seahorse Bioscience | ||
| Other | BD FACSAria II SORP | Becton Dickinson | RRID:SCR_018091 | Flow cytometry sorting |
| Other | Illumina Nextseq500 platform | Illumina | RRID:SCR_014983 | RNAseq |
| Other | Biograph 40 mCT scanner | Siemens Healthineers | ~2.1 MGq/kg FDG i.v. | FDG PET CT |