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. 2020 Oct 22;7(5):ENEURO.0194-20.2020. doi: 10.1523/ENEURO.0194-20.2020

Figure 4.

Figure 4.

Topographical organization D1R+ and D2R+ neurons projecting to DMS and DLS. PFC sections from D1Cre (Ai) and D2Cre (Bi) mice showing cell bodies retrograde labeled with either GFP or TdTomato, respectively. Left panels, Zoomed insets of medial PrL and MO showing layer-specific localization of cell bodies. PrL, prelimbic; Cg1, cingulate; MO, medial orbitofrontal; AI, agranular insular; M1, primary motor; M2, secondary motor cortex. Aii, Bii, Injection sites are shown in DMS and DLS subregions in striatal sections. D1Cre and D2Cre mice were injected with Cre-dependent retrograde AAV, AAVRG-CAG-Flex-TdTomato, and AAVRG-hSYN-DIO-GFP in DMS (50 nl) and DLS (50 nl). Representative images (n = 5). C, D, Quantification of number of cells from D1Cre and D2Cre mice. E, F, Comparison of number of labeled cells in PFC of D1 and D2Cre mice projecting to DMS and DLS subregions. Data are represented as mean ± SEM; *p < 0.05, **p < 0.01, two-way ANOVA. Scale bar: 100 μm.