Table 1.
Strains and plasmid used in this study.
| Strains or plasmids | Genotypic or construct | References or origin |
|---|---|---|
| AY15 CICC32315 | Commercial Baijiu yeast strain | This lab |
| α5 | MATα, haploid yeast strain from AY15 | This lab |
| Transformants | ||
| △GAT1 | α5△GAT1::loxP-KanMX-loxP | This study |
| △AGP1 | α5△AGP1::loxP-KanMX-loxP | This study |
| △BAP2 | α5△BAP2::loxP-KanMX-loxP | This study |
| △GDH1 | α5△GDH1::loxP-KanMX-loxP | This study |
| △ARO80 | α5△ARO80::loxP-KanMX-loxP | This study |
| △CAN1 | α5△CAN1::loxP-KanMX-loxP | This study |
| △BAT2 | α5△BAT2::loxP-KanMX-loxP | This study |
| △GAD1 | α5△gad1::loxP-KanMX-loxP | This study |
| Plasmid | ||
| pUG6 | Kanr, containing loxP-KanMX-loxP disruption cassette | Güldener et al., 2002 |