Table 2.
Results of the bacterial reverse mutation test for water-extract sericin.
| Groups | TA97a | TA98 | TA100 | TA102 | TA1535 | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | |
| Sericin | ||||||||||
| 5000 μg/plate | 117.0 ± 17.7 | 125.0 ± 17.5 | 38.3 ± 4.5 | 39.0 ± 4.4 | 157.3 ± 21.7 | 155.3 ± 18.6 | 269.0 ± 29.5 | 277.7 ± 22.0 | 24.0 ± 5.3 | 21.7 ± 5.5 |
| 1581 μg/plate | 131.3 ± 25.0 | 127.3 ± 22.0 | 39.7 ± 4.2 | 42.3 ± 4.2 | 150.7 ± 19.6 | 156.0 ± 16.7 | 280.0 ± 20.1 | 273.0 ± 20.1 | 18.3 ± 2.3 | 22.3 ± 5.9 |
| 500 μg/plate | 124.7 ± 19.7 | 114.7 ± 18.7 | 38.7 ± 7.2 | 41.7 ± 6.5 | 154.0 ± 19.7 | 152.0 ± 20.5 | 271.7 ± 22.1 | 266.7 ± 23.9 | 22.7 ± 3.2 | 26.7 ± 9.2 |
| 158 μg/plate | 123.7 ± 21.2 | 118.7 ± 23.7 | 37.7 ± 4.7 | 41.0 ± 4.4 | 152.7 ± 17.8 | 159.7 ± 24.0 | 275.7 ± 22.0 | 275.3 ± 21.2 | 22.3 ± 7.1 | 24.3 ± 4.2 |
| 50 μg/plate | 125.7 ± 16.0 | 120.3 ± 17.5 | 43.3 ± 5.0 | 43.0 ± 7.6 | 151.3 ± 21.5 | 156.7 ± 20.5 | 267.7 ± 22.9 | 275.7 ± 21.0 | 22.7 ± 7.0 | 25.0 ± 6.2 |
| Untreated control | 120.0 ± 13.2 | 130.0 ± 10.5 | 41.3 ± 5.5 | 44.3 ± 6.7 | 148.3 ± 22.3 | 155.3 ± 25.6 | 271.7 ± 21.2 | 274.7 ± 21.0 | 23.3 ± 6.8 | 23.7 ± 4.5 |
| H2O control | 125.0 ± 15.6 | 127.0 ± 19.3 | 41.0 ± 5.0 | 38.0 ± 4.6 | 157.3 ± 19.7 | 150.7 ± 19.4 | 271.0 ± 20.5 | 269.7 ± 19.8 | 21.7 ± 8.3 | 24.3 ± 5.8 |
| DMSO control | 124.3 ± 17.2 | 122.3 ± 17.6 | 36.0 ± 5.3 | 38.0 ± 7.0 | 157.7 ± 22.2 | 158.0 ± 21.6 | 279.3 ± 19.9 | 271.3 ± 16.3 | 20.0 ± 4.4 | 25.3 ± 8.3 |
| Positive control | ||||||||||
| 2-AF | 1785.3 ± 68.2 | 4886.7 ± 210.1 | 2886.7 ± 102.6 | |||||||
| DNR | 2980.0 ± 111.4 | |||||||||
| Dexon | 2795.3 ± 60.5 | 864.7 ± 68.5 | ||||||||
| NaN3 | 3056.7 ± 156.3 | |||||||||
| 1,8-DHAQ | 864.3 ± 73.6 | |||||||||
| CP | 308.7 ± 13.3 | 139.3 ± 14.7 | ||||||||
Values represent the mean ± standard deviation of triplicates. -S9: without metabolic activation; +S9: with metabolic activation; 2-AF: 2-aminofluorene (10 μg/plate); DNR: daunorubicin (6 μg/plate); Dexon: dexon (50 μg/plate); NaN3: sodium azide (1.5 μg/plate); 1,8-DHAQ: 1, 8-dihydroxyanthraquinone (50 μg/plate); CP: cyclophosphamide (200 μg/plate).