. 2020 Nov 6;2020:2793804. doi: 10.1155/2020/2793804

Table 3.

Effects of diabetes (DM) and/or estrogen deficiency on the bone mass, macrometric parameters, composition, and mineralization in the femur and L4 vertebra.

Parameter/group		Nonovariectomized (NOVX) rats		Ovariectomized (OVX) rats		Two-way ANOVA
Parameter/group		Control	DM	Control	DM	OVX	DM	OVXx DM
Bone mass (g)	Femur	0.682 ± 0.014	0.629 ± 0.012^∗∗	0.677 ± 0.014	0.618 ± 0.012^∗∗^XX	NS	p < 0.001	NS
Bone mass (g)	L4 vertebra	0.214 ± 0.009	0.188 ± 0.008^∗	0.214 ± 0.006	0.178 ± 0.005^∗∗∗^XX	NS	p < 0.001	NS
Femoral length (mm)		33.54 ± 0.18	32.83 ± 0.16^∗	34.07 ± 0.22	32.80 ± 0.20^∗∗^XXX	NS	p < 0.001	NS
Femoral diameter (mm)		3.36 ± 0.03	3.28 ± 0.03	3.37 ± 0.04	3.26 ± 0.04	NS	p < 0.01	NS
Bone mineral mass (g)	Femur	0.331 ± 0.007	0.293 ± 0.007^∗∗∗	0.320 ± 0.006	0.283 ± 0.005^∗∗∗^XXX	NS	p < 0.001	NS
Bone mineral mass (g)	L4 vertebra	0.086 ± 0.003	0.073 ± 0.003^∗∗∗	0.086 ± 0.002	0.068 ± 0.002^∗∗∗^XXX	NS	p < 0.001	NS
Bone mineral content (g/g)	Femur	0.485 ± 0.003	0.466 ± 0.006^∗∗	0.473 ± 0.004^∗	0.458 ± 0.003^∗∗∗^XX	p < 0.05	p < 0.001	NS
Bone mineral content (g/g)	L4 vertebra	0.407 ± 0.011	0.391 ± 0.008	0.403 ± 0.007	0.384 ± 0.005	NS	p < 0.05	NS
Bone water content (g/g)	Femur	0.276 ± 0.004	0.294 ± 0.007	0.303 ± 0.013	0.301 ± 0.004	p < 0.05	NS	NS
Bone water content (g/g)	L4 vertebra	0.345 ± 0.014	0.362 ± 0.010	0.347 ± 0.007	0.362 ± 0.009	NS	NS	NS
Bone organic substances content (g/g)	Femur	0.239 ± 0.002	0.240 ± 0.002	0.224 ± 0.012	0.241 ± 0.002	NS	NS	NS
Bone organic substances content (g/g)	L4 vertebra	0.248 ± 0.004	0.248 ± 0.003	0.250 ± 0.002	0.254 ± 0.006	NS	NS	NS
Calcium content (g/g of bone mineral)	Femur	0.373 ± 0.007	0.364 ± 0.012	0.363 ± 0.007	0.377 ± 0.006	NS	NS	NS
Calcium content (g/g of bone mineral)	L4 vertebra	0.345 ± 0.018	0.347 ± 0.016	0.346 ± 0.015	0.339 ± 0.015	NS	NS	NS
Phosphorus content (g/g of bone mineral)	Femur	0.176 ± 0.003	0.173 ± 0.002	0.171 ± 0.002	0.180 ± 0.002^{XX a}	NS	NS	p < 0.01
Phosphorus content (g/g of bone mineral)	L4 vertebra	0.156 ± 0.004	0.155 ± 0.003	0.158 ± 0.004	0.153 ± 0.003	NS	NS	NS

Bilateral ovariectomy was performed 5 weeks before the end of the experiment. Diabetes was induced 3 days after the ovariectomy by a single administration of streptozotocin (60 mg/kg i.p.). Results are presented as means ± SEM (n = 9 − 10). Two-way ANOVA followed by Fisher's LSD test was used for evaluation of the significance of the results. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001—significantly different from the NOVX control rats; ^XXp < 0.01, ^XXXp < 0.001—significantly different from the OVX control rats; ^ap < 0.05—significantly different from the DM-NOVX rats.