Skip to main content
. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Adv Healthc Mater. 2020 Sep 23;9(21):e2000880. doi: 10.1002/adhm.202000880

Figure 2.

Figure 2.

Characteristics of HMVEC monolayer in L-TumorChip at day 2 post-seeding. (A) Representative phase-contrast image of the confluent HMVEC endothelium monolayer. Representative CD-31 immunostained images with (B) 10× and (C) 20× magnifications under the confocal microscope. Images are a maximum intensity projection of the full z-stack images captured from bottom to top layers. Representative images of the endothelium stainted with VE-cadherin antibody with (D) 10× and (E) 20× magnifications under the confocal microscope, focusing on slices above and proximal to the middle layer. The slices obtained from this subset of layers is shown as a maximum intensity projection. (F) Permeability of HMVEC barrier in L-TumorChip under the interaction with MDA-MB-231 cancer cells. Cancer cells were tagged with GFP in (B) and (C) or RFP in (D) and (E). Hoechst dye was used to stain nuclei. Results are represented as average ± S.D. (n = 4). Significance was determined using 2-way ANOVA and Tukey’s Multiple Comparison Test (**, p < 0.01).