Skip to main content
. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Am J Transplant. 2020 Jun 11;21(1):73–86. doi: 10.1111/ajt.16004

Figure 6. Tfr suppression of Tfh:B cell interactions is not CTLA-4-dependent in the presence of selective CD28 blockade.

Figure 6.

(A) Naïve B6 mice were transplanted with BALB/c skin, left untreated, and sacrificed 10 days post-transplant. Tfh, Tfr and B cells from graft-DLNs were isolated and co-cultured for 5 days. Tfh:B and Tfh:B + Tfr cell co-cultures were left untreated or treated with anti-CTLA-4 mAb alone, anti-CD28 dAb alone, or anti-CD28 dAb plus anti-CTLA-4 mAb. (B) Representative flow plots from B cell alone (control) and Tfh:B cell co-cultures depicting class-switched GC-like GL7+IgG1+ B cells under the indicated conditions. Summary data of the fold change in frequency of these class-switched B cells relative to B cell alone controls (n=4–9 per group). (C) Summary data of supernatant total IgG levels (n=4–9 per group). (D) Representative flow plots from Tfh:B + Tfr cell co-cultures depicting GL7+IgG1+ B cells under the indicated conditions. Summary data of the fold change in frequency of these class-switched B cells relative to B cell alone controls (n=3–6 per group). (E) Summary data of supernatant total IgG levels (n=3–6 per group). Summary data represent mean (SE) and are combined from 2 independent experiments, with cells pooled from 10 mice per experiment and 3–9 co-culture wells per treatment group. *p < 0.05, **p < 0.01.