Table 2.
Examples of engineered PTases to enhance or alter specificity and activity.
| Enzyme | Mutation | Product Profile | Reference |
|---|---|---|---|
| FgaPT2 | K174F | Loss of activity towards Trp, increase towards Tyr | [93] |
| K174F:R244Y | Increase towards indole DKPs | [94] | |
| M328G | Increase towards GPP and away from DMAPP | [95] | |
| R244Q:M328G | Increase towards GPP and away from DMAPP, accept indole DKPs | [75] | |
| FtmPT1 | M364G | Increase towards GPP and away from DMAPP | [75] |
| CdpC2PT | T351G | Increase towards GPP and away from DMAPP | [75] |
| CdpC3PT | F335G | Increase towards GPP and away from DMAPP | [75] |
| CdpNPT | M349G | Increase towards GPP and away from DMAPP | [75] |
| BrePT | I337G | Increase towards GPP and away from DMAPP | [75] |
| A173M | Increase towards DMAPP and away from GPP | [96] | |
| TleC | W97Y | Increase towards DMAPP | [96] |
| MpnD | Y80W | Increase towards GPP and away from DMAPP | [96] |
| M159A | Increase towards GPP/FPP and away from DMAPP | [96] | |
| PagF | F222A | Increase towards GPP and away from DMAPP | [97] |
| AtaPT | W397A | Altered diversity of many acceptor compounds | [76] |
| E91A/E91Q | Only monoprenylations for all wild-type products | [76] | |
| EpzP | A285Q | 14-fold increase in enzyme velocity | [98] |