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. Author manuscript; available in PMC: 2021 Nov 12.
Published in final edited form as: J Med Chem. 2020 Sep 10;63(21):12137–12155. doi: 10.1021/acs.jmedchem.0c00724

Figure 7. Interference profiling of CBD.

Figure 7

(A) CBD shows moderate activity at several receptors. Ki values reported from testing by PDSP. Full data in Supporting Information. (B) CBD shows concentration-dependent, detergent-sensitive inhibition of AmpC in a colloidal aggregation counter screen. TIPT, positive aggregation control; 2-BTBA, positive non-aggregator control. Data are mean SD of four intra-run technical replicates. (C) CBD shows detergent-sensitive inhibition of MDH in an orthogonal aggregation counter screen. Compounds were tested at 33 or 100 μM final concentrations in either the presence (blue) or absence (magenta) of buffer containing freshly-added 0.01% Triton X-100 (v/v). Data are mean SD of at least three intra-run technical replicates. (D) CBD forms detectible colloidal aggregates at approximately 12.5 μM by DLS. (E) CBD does not produce detectable H2O2 in a HRP-PR redox-cycling counter screen. Compounds assayed at 250 μM final concentrations 1 mM DTT, enzyme. H2O2, positive control; NSC-663284 and 4-amino-1-naphthol, positive control compounds. Data are mean SD of at least three intra-run technical replicates.