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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Hypertension. 2020 Nov 2;76(6):1744–1752. doi: 10.1161/HYPERTENSIONAHA.120.15435

Figure 1: Effects of TNF on renal cortical AGT mRNA and protein expression during LS intake.

Figure 1:

Mice received LS [0.02% NaCl (wt/wt)] or NS [0.4% NaCl (wt/wt)] for 7 days. A): AGT mRNA expression was measured by RT-PCR after mice ingested LS or NS for 7 days. B): Western blotting of AGT using anti-AGT antibody (top); anti-β-actin antibody was used as a loading control (bottom). C and D): AGT mRNA and protein expression in renal cortex was measured by RT-PCR or Western blotting, respectively, after intrarenal injection of either ~5 × 107 transducing units (TU) lentivirus U6-TNF-ex4 or U6 control constructs into both kidneys of mice that ingested LS for 7 days. E and F): AGT mRNA and protein expression was measured after a single intrarenal injection of murine recombinant TNF-α (5 ng/g body wt) into both kidneys followed by LS for 7 days. Data are shown as mean ± SE; (n=10).