The healing effects of lyophilized and fresh cMSCs-derived conditioned-medium compared with HCLE-derived conditioned-medium and unconditioned media (control) on in vitro scratch (wound healing) assay of HCLE cells. The representative images (A) show faster wound closure in wounds treated with lyophilized and fresh cMSC-derived conditioned-medium compared with HCLE-derived conditioned-medium and control. Quantitative comparison (B) showed that after 17 hours, wounds treated with fresh and lyophilized cMSCs’ conditioned-medium were closed by 82.2 ± 6.1% and 86.3 ± 7.2%, respectively, while HCLE-derived conditioned-medium and control wounds were closed by 54.1±7.6% and 49.1 ± 9.3%, respectively. There was no significant difference between fresh and lyophilized cMSCs’ conditioned-medium (****, P<0.0001, ns, Not Significant, N=3 wells per each group).