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. 2020 Nov 13;3:672. doi: 10.1038/s42003-020-01397-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A biotinylated human or murine derived phosphopeptide was immobilized onto a streptavidin-coated gold sensor chip for a surface plasmon resonance-based assay. On-rates were determined by applying an analyte solution of purified 6xHis tagged HACS1 SH3 domain or SHP1 N-SH2 domain at the indicated concentrations. Off-rates were determined by switching to an application of buffer alone at 120 s. Curve fits were made according to a 1:1 interaction model.