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. 2020 Nov 13;10:19791. doi: 10.1038/s41598-020-76139-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative analysis of accessory gland gene sets with mosquitoes and midges to the Antarctic midge. (A) Female accessory gland genes compared to midges (left), mosquitoes (middle), and genes with enriched expression in the female reproductive tract of mosquitoes (right)²⁵. (B) Male accessory gland genes compared to midges (left), mosquitoes (middle), and genes with enriched expression in the male reproductive tract of mosquitoes (right)²⁵. (C) Overlap between genes expressed in male accessory glands between mosquitoes and B. antarctica. Left, highly enriched in Anopheles male accessory gland. Right, enriched in Anopheles male accessory gland. Enrichment for Anopheles male accessory gland genes is based on values from Izquierdo et al.²⁶. Protein sequences were defined as orthologs if they had reciprocal-best BLASTp hits with an e-value < 10⁻¹⁰.