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. 2020 Nov 13;10:19817. doi: 10.1038/s41598-020-76882-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Leukocyte infiltration and inflammation-related mRNA levels in corneal grafts. (A) Representative flow cytometry plots and (B) statistical analysis of flow cytometry data showing the frequency of occurrence of infiltrating CD45⁺ leukocytes in grafted corneas at day 14 post-transplantation (n = 3, one-way ANOVA; *p < 0.05). mRNA levels of Cd11b (C) and Cd11c (D) expressed on the surface of leukocytes, including neutrophils, macrophages, and dendritic cells, in grafted corneas on day 14 post-transplantation (Cd11b n = 3, one-way ANOVA, **p < 0.01, ***p < 0.001; Cd11c n = 3, one-way ANOVA, ***p < 0.001). (E) mRNA levels of inflammation-related markers in grafted corneas on day 14 post-transplantation (n = 3, one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001). (F) mRNA level of Interleukin (Il)10 in the grafted cornea on day 14 post-transplantation (n = 3, one-way ANOVA; **p < 0.01, ***p < 0.001). Statistical analyses of flow cytometry data were performed using FlowJo software X 10.5.3. (FlowJo LLC, Ashland, OR, USA; purchased from https://www.flowjo.com). ANOVA analysis of variance, Ifnγ Interferonγ, Tnf-α Tumor necrosis factor-α.