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. 2020 Nov 13;10:19814. doi: 10.1038/s41598-020-76391-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme describing how the experiments for the bile duct ligation (BDL) animal model were performed. Surgery was done on day 0 and the distress parameters were evaluated during pre, acute, early, middle and late phase on the indicated days. Therapies with MCC950 or vehicle solution were performed by daily subcutaneous injection from day − 1 to day 13.