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. 2020 Nov 13;10:19814. doi: 10.1038/s41598-020-76391-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distress of survivors and non-survivors after BDL. The distress score (a) was increased (Mann–Whitney rank sum test, P ≤ 0.001), whereas burrowing activity (b) was decreased (Mann–Whitney rank sum test, P ≤ 0.001) and body weight (c) was also reduced (Mann–Whitney rank sum test, P = 0.031), when comparing data of survivors (sur.) to data of non-survivors (non-sur.). ROC curve analysis shows the area under the curve (AUC) for single (d), two (e) or all three (f) distress parameters. The performance of single and multiple parameters is described by presenting the AUC, the 95% confidence interval (CI) and the asymptotic P-value (g). Survivors: 16 mice, 48 data points; non-survivors: 10 mice, 10 data points.