Figure 5.

Distress of mice after CCl₄ injections and after bile duct ligation. Scheme describing how the experiments for the CCl₄ animal model were performed (a): CCl₄ was injected sc on the indicated days, the distress parameters were evaluated during pre, acute, early, middle and late phase and a therapy with MCC950 or vehicle solution was performed as daily subcutaneous injections from day 28 to 39. The distress score (b) was decreased (Mann–Whitney rank sum test, P ≤ 0.001), whereas burrowing activity (c) was increased (Mann–Whitney rank sum test, P ≤ 0.001) and body weight loss (d) was decreased (Student’s t test, P ≤ 0.001) when comparing post-BDL to post-CCl₄ animals. BDL: 16 mice = 48 data points; CCl₄: 10 mice = 30 data points.