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. 2020 Nov 13;11(11):979. doi: 10.1038/s41419-020-03173-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Overlay of fluorescent and merged phase images of a CD8⁺ T-cell co-encapsulated with a dendritic cell before and during a calcium peak. CD⁺ T cells are fluorescent and dendritic cells are unlabeled. B Representative normalized fluorescent intensity profile of a single cell displaying with calcium peak highlighted in green. C Average calcium peaks for each CD8⁺ T cells over 4.5 h. The sample sizes (n) for this experiment are: naive: 35, partially activated control: 21, PD1: 45, LAG3: 24, and PD1⁺ LAG3: 15 cells.