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. 2020 Nov 13;10:19825. doi: 10.1038/s41598-020-76972-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sequencing metrics. Panels a-d show the effect of read depth on (a) sequencing saturation, (b) total genes detected, (c) median genes per cell and (d) median unique molecular identifier (UMI) counts per cell. Panels e and f show cell numbers pre and post quality control for (e) unstimulated and (f) stimulated samples. Unstimulated datasets are shown in blue and stimulated datasets in red.