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. 2020 Nov 13;10:19825. doi: 10.1038/s41598-020-76972-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Confusion matrices comparing cell type classification between full sequencing depth and low/high subsampled depths. Tiles are shaded and numbered according to the number of cells classified and diagonal tiles represent concordant cell type classification between depths. Unstimulated sample (a) 261,082 versus 66,744 reads per cell and (c) 261,082 versus 197,421 reads per cell. Stimulated sample (b) 333,333 versus 67,604 reads per cell and (d) 333,333 versus 248,770 reads per cell.