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. 2020 Nov 13;11:5772. doi: 10.1038/s41467-020-19638-4

Fig. 1. Adult-onset ciliary dysgenesis in POMC neurons does not change energy and glucose metabolism.

Fig. 1

a Experimental scheme in mice with POMC-specific IFT88 depletion that was induced by tamoxifen injections at 7 weeks. b Representative confocal images of AC3 (adenylyl cyclase-3, cilia marker) and tdTomato double staining in the ARH of POMC-cre/ERT2;;IFT88f/f;;tdTomato mice and POMC-cre/ERT2;;tdTomato mice (n = 5). Arrowheads indicate cilia of POMCtdTomato neurons. The graph depicts the average cilia lengths of about 100 POMC and non-POMC neurons. *p < 0.001. Scale bars: 20 μm. c Body weights in POMC-cre/ERT2;;IFT88f/f mice and their IFT88f/f littermates on a chow diet (n = 10 for IFT88f/f mice, n = 8 for POMC-cre/ERT2;;IFT88f/f mice). d Lean mass and fat mass measured at 12 weeks (n = 10 for IFT88f/f mice, n = 7 for POMC-cre/ERT2;;IFT88f/f mice). e Average weekly food intake during 4–16 weeks (n = 5). f Energy expenditure measured at 12 weeks (n = 5). g Glucose and insulin tolerance tests performed at 15 weeks (n = 7). h Body weights and cumulative food intake during a high-fat diet (HFD) challenge (n = 7). Data are presented as the mean ± SEM values. Statistics were performed using two-sided Student’s t test (b, d) and one-sided two-way ANOVA (c, e–h) followed by post hoc least significant difference (LSD) test. ns not significant.