Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 13;11(11):977. doi: 10.1038/s41419-020-03186-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A Quantitative PCR analysis of PTEN in shNC or shYTHDC1-transfected neurons subjected to OGD for 1 h or under normal conditions (Ctrl). β-actin served as a loading control. B The qRT-PCR analysis of PTEN mRNA levels in shNC or shYTHDC1-transfected neurons under normal conditions after ActD (1 µg/ml) treatment for the indicated times. C The qRT-PCR analysis of PTEN mRNA levels in shNC or shYTHDC1-transfected neurons subjected to ActD (1 µg/ml) treatment for the indicated times after OGD for 1 h. D RIP qRT-PCR analysis of PTEN and β-actin in YTHDC1-RNA complexes. Data are presented relative to control immunoprecipitation of protein-RNA with immunoglobulin G (IgG). E Dual-luciferase reporter assay in lysates of PC12 cells transfected with luciferase reporter plasmids for 3′-UTR of PTEN plus YTHDC1 or Vector. Results are relative to Renilla luciferase activity. The data are means ± S.E.M., for all panels: *P < 0.05, **P < 0.01, ***P < 0.001. B, C, E used student’s t test; A and D used two-way ANOVA analysis. All data are representative of or combined from at least three independent experiments.