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Cell-free synthesis of WT-HV1 and Mel-HV1 in K562 cell lysate system: total protein yield and secreted/released amount of hirudin (SN2 fraction) in nmol/l and µg/ml; ratio between total protein yield and secreted/released amount of hirudin represents the secretion efficiency.
| Total yield | Secreted amount | Ratio (%) | |
|---|---|---|---|
| WT-HV1 | 1245 nmol/l | 158 nmol/l | 12.7 |
| 8.8 µg/ml | 1.1 µg/ml | ||
| Mel-HV1 | 6150 nmol/l | 509 nmol/l | 8.3 |
| 43.2 µg/ml | 3.6 µg/ml |
Determination was performed by double determination (n = 2).
