Fig. 3.

Expresssion of small heat shock proteins. a Relative protein expression of Hsp27, αB-crystallin, Hsp20 and actin detected by Western Blot analysis in WT, R33Q KI, D307H KI and KO hearts. On the right, average percentages of R33Q CASQ2, D307H CASQ2 and KO are given as mean ± SE, n = 4, and are compared to WT expressed as 100% ± SE. b Relative protein expression of phosphorylated forms of Hsp27 (phosphoSer82), αB-crystallin (phosphoSer59 and phosphoSer45), Hsp20 (phosphoSer16) and actin in WT, R33Q KI, D307H KI and KO hearts. On the right, average percentages of R33Q CASQ2, D307H CASQ2 and KO are given as mean ± SE (n = 4) and are compared to WT expressed as 100% ± SE. Asterisks (*) indicate a p < 0.05 as determined by one-way ANOVA Bonferroni’s test. Blots are representative images of each group and each experiment was repeated at least thrice