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. 2020 Sep 14;22(6):1523–1531. doi: 10.1007/s11307-020-01523-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1. — (a) Bioluminescence profiles for Luc2, CBR2, CBG99, and Akaluc luciferases combined with four different luciferin analogues in live cells. (b-f) Photon flux (ph/s) in HEK 293T cells expressing individual luciferases upon addition of substrates (0.1 mM) was quantified using an exposure time of 30 s. Statistical analysis (N = 3) was performed using one-way ANOVA followed by Tukey’s T test (*p < 0.01 for Luc2/d-LH2 compared with all combinations with the exception of Akaluc/Cycluc1 which was not significantly different).