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. 2020 Sep 14;22(6):1523–1531. doi: 10.1007/s11307-020-01523-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2. — In vivo (BALB/C) emission spectra for different combinations of luciferase (Luc2, CBG99, CBR2, or Akaluc; expressed in HEK393T cells implanted subcutaneously in the flanks) and luciferin or luciferin analogue. (a) D-LH2 (150 mg/kg), (b) NH₂-NpLH2 (220 mg/kg), (c) CycLuc1 (7.6 mg/kg), and (d) Akalumine-HCl (50 mg/kg); substrates were injected intraperitoneally). Spectral data was acquired 15–20 min after injection.