Fig. 5.
Aβ isoform composition of the coarse-grained plaque compared to the cotton wool and classic cored plaque. a, b The coarse-grained plaque was predominantly Aβ40 immunoreactive compared to Aβ42. c, d Strong immunostaining was also seen for AβN3pE and pSer8Aβ. This Aβ composition differed from that of the cotton wool plaque (e–h) and the classic cored plaque (i–l). e, f The cotton wool plaque showed a central staining for Aβ40, that was surrounded by an Aβ42 immunoreactive ring. g, h The cotton wool plaque was immunoreactive for AβN3pE and displayed a distinct halo immunoreactive for pSer8Aβ. i, j Classic cored plaques were predominantly Aβ42 compared to Aβ40 immunoreactive. k, l Both AβN3pE and pSer8Aβ were detected in the entire classic cored plaque. Scale bar represents 50 µm and is applicable to all images. Aβ40 amyloid-beta 40, Aβ42 amyloid-beta 42, AβN3pE truncated pyroglutamate Aβ, pSer8Aβ phosphorylated Aβ at serine 8