Table 1.
- Antibodies used, characterized by clone, laboratory, dilution and pH.GIP: gastrin inhibitor peptide; Tryptase: mast cell-related tryptase; VEGFA: vascular endothelial growth factor; VIP: intestinal vasoactive peptide; TNFα: tumor necrosis factor alpha; IL-1: interleukin 1; PGE-2: prostaglandin E 2; PGP 9.5: gene-protein product 9.5; CD8: CD8 T lymphocytes.
| Antibody | Clone | Laboratory | Dilution | pH |
|---|---|---|---|---|
| GIP | 4 | Enzo | 1:75 | 6 |
| Tryptase | AA1 | DAKO | 1:200 | 6 |
| VEGFA | Policlonal | ABCAM | 1:200 | 9 |
| VIP | Policlonal | ABCAM | 1:50 | 9 |
| TNFα | 2C8 | FITZGERALD | 1:100 | 6 |
| Enolase | BBS/NC/VI-H14 | DAKO | No dilution | 9 |
| IL-1 | Policlonal | ABCAM | 1:800 | 6 |
| PGE-2 | Policlonal | BIOSS | 1:200 | 9 |
| PGP 9.5 | 3D9 | FITZGERALD | 1:500 | 6 |
| CD8 | C8/144B | DAKO | No dilution | 9 |
| S-100 protein | 4C4.9 | DAKO | No dilution | 9 |
| Synaptophysin | DAK-SYNAP | DAKO | No dilution | 6 |