Figure 1: Ex vivo expansion results in CD3⁺Vα24⁺Vβ11⁺, predominantly CD4⁺ iNKT cells.

(A) Median and IQR ± range absolute number CD3⁺Vα24⁺ iNKT cells at day 0 and day 7 (N = 47) (left upper panel) and representative flow plots of CD3 and Vα24 expression (right upper panel). Median and IQR ± range absolute number CD3⁺Vα24⁺ iNKT cells after day 7 sort, including days 14, and 21 (N = 47) (left lower panel) with representative flow plots showing CD3 and Vα24 expression pre- and post-sort on gated CD3⁺ cells on day 7 (right lower panel).

(B) Left panel, median absolute number CD3⁺Vα24⁺ iNKT cells at day 7, day 14, and day 21 stratified by day 7 sort yield categories 10⁴-10⁵ (orange, N = 4), 10⁵-10⁶ (yellow, N = 16), 10⁶-10⁷ (cyan, N =21), and >10⁷ (magenta, N = 6). Right panel, sub analysis of expansion data by day 7 sort categories showing median and IQR ± range absolute number CD3⁺Vα24⁺ iNKT cells at specified time points in the expansion protocol (S, day 7 post-sort).

(C) Median and IQR ± range percentage (left panel) with representative flow plots of CD4 and CD8 expression in gated CD3⁺Vα24⁺ cells on days 0, 7, and 21 (N = 36). Median and IQR ± range absolute number of CD4⁺, DN, and CD8⁺ CD3⁺Vα24⁺ iNKT cells on days 0, 7, and 21 (left panel; N = 36). P < 0.05 is represented by *.