Table 1.

INKT cell expansion protocols

First Author	aGalCer/PHA	iNKT cell isolation	Cytokine	Feeders/APC
Watarai	aGalCer	No pre-enrichment	IL-2	Auto APC (GM-CSF + IL-4)
Schmid	KRN7000,PBS44, PBS57	No pre-enrichment	IL-2	Auto PBMC
Moreira-Teixeira	aGalCer or OCH	No pre-enrichment	TGFb, +/− Rapamycin	Auto PBMC
Nishi	aGalCer	No pre-enrichment	IL-2, IL-15 and IL-7	Auto PBMC
Van Der Vliet	aGalCer	No pre-enrichment	IL-12 or IL-7	Auto PBMC
Van der Vliet	aGalCer	No pre-enrichment or Mag bead	IL-15 or IL-7 +dexamethasone	Auto PBMC or Auto mo-DC
van der Vliet	aGalCer	Monocyte depletion	IL-7 and/or IL-15	B cells or EBV transformed B-LCLs or moDC (IL4+GM-CSF)
Li	aGalCer	Neg fraction CD14-Mag Beads	IL-2	CD14+ fraction (GM-CSF + IL-4)
East	aGalCer	CD3+CD161+Mag Beads	IL-2	Artificial APC
Exley	aGalCer	FACS sorting	IL-2	Auto Irradiated PBMC
O’Reilly	PHA	Mag beads + sorting	IL-2	Allo PBMC
Zeng	PHA	Mag beads + sorting	IL-2	Plate bound anti-CD3 or Allo feeders
Takahashi	aGalCer	Mag beads	IL-2	Auto Mo-DC (IL-4 GM-CSF)
Tian	aGalCer	Microbeads	IL-2	Auto PBMC, transduced K562 line
Song	aGalCer	Mag beads	IL-2	APC (GM-CSF +IL-4 + LPS)
Metelitsa	aGalCer	Mag beads	IL-2	Auto Vα24-neg fraction
Huijts	aGalCer	Mag beads	IL-2	Mature Mo-DC
Trujillo-Ocampo	aGalCer	Mag beads	IL-2	Allo monocyte derived DCs
Rogers	aGalCer	Mag beads at Day 12	IL-2	Auto PBMC
Mavers	aGalCer	Mag beads	IL-2 or IL-2 + IL-15	Auto PBMC
Moreno	aGalCer	Mag beads	IL-2, IL-7, IL-15	Auto PBMC
Moreno	aGalCer	Mag beads	IL-2, rhIL-7, IL-15	Mature M3CD1d-DC
Motohashi	aGalCer	No pre-enrichment	IL-2	Auto PBMC
Kunii	aGalCer	No pre-enrichment	IL-2	Auto PBMC
Yamasaki	aGalCer	No pre-enrichment	IL-2	Auto PBMC
Exley	None	CliniMACS	IL-2	anti-CD3 coated 24 well plates + auto PBMC