Fig. 6.

ZLM-7 reduced VEGFA expression through miR-212-3p/SP1 and inhibited tumor growth and angiogenesis. a Picture of tumors in mice model. b Tumor growth curve in indicated groups. c Average body weight of mice tumors in indicated groups. d The expression of miR-212-3p, Sp1 and VEGFA in tumor tissues of mice treated with ZLM-7 and miR-212-3p inhibitor was detected by qRT-PCR. e, f Western blot detected Sp1, VEGFA and MMP2 protein levels of tumor tissues in indicated groups. Relative intensity of bands of each protein were normalized to loading control GAPDH. g IHC analysis of tumor tissues in indicated groups. *P < 0.05, **P < 0.01, ***P < 0.001