Table 1.
Antibodies used in this study
| Antibody | Fluorophore | Clone | Usage | Manufacturer |
|---|---|---|---|---|
| Rat anti-mouse CD16/CD32 | N/A | 2.4G2 | Fc block | BD Biosciences |
| Mouse anti-mouse CD64 | PE | X54-5/7.1 | Eye | BioLegend |
| Hamster anti-mouse CD11c | BV 421 | HL3 | Eyea | BD Biosciences |
| Rat anti-mouse Ly6G | PE-CF594 | 1A8 | Eye | BD Biosciences |
| Mouse anti-mouse NK1.1 | PE-CF594 | PK136 | Eye | BD Biosciences |
| Rat anti-mouse Siglec F | PE-CF594 | E50-2440 | Eye | BD Biosciences |
| Rat anti-mouse B220 | PE-CF594 | RA3-6B2 | Eye | BD Biosciences |
| Rat anti-mouse CD8 | PE-CF594 | 53-6.7 | Eye and blood | BD Biosciences |
| Rat anti-mouse CD4 | PE-CF594 | RM4-5 | Eye and blooda | BD Biosciences |
| Rat anti-mouse MHC II | AlexaFluor 700 | M5/114.15.2 | Eye | BioLegend |
| Rat anti-mouse CD11b | APC-Cy7 | M1/70 | Eyea | BD Biosciences |
| Rat anti-mouse CD45 | PE-Cy7 | 30-F11 | Eye and blooda | BD Biosciences |
| Rat anti-mouse Ly6G | PerCP-Cy5.5 | 1A8 | Blooda | BD Biosciences |
| Rat anti-mouse CD11b | eFluor 450 | M1/70 | Blooda | Invitrogen |
| Rat anti-mouse CD19 | APC | 1D3 | Blooda | BD Biosciences |
| Mouse anti-mouse NK1.1 | AlexaFluor 700 | PK136 | Blood | BD Biosciences |
| Rat anti-mouse CD115 | PE | AFS98 | Blood | Invitrogen |
| Rat anti-mouse CD19 | PE | 1D3 | Compensation | BD Biosciences |
| Rat anti-mouse CD19 | AlexaFluor 700 | 1D3 | Compensation | BD Biosciences |
| Fixable viability dye | eFluor 506 | N/A | Eyea | Invitrogen |
| Rat anti-mouse CD102 (ICAM2) | N/A | 3C4(mIC2/4) | Immunofluorescence | BD Biosciences |
| Rabbit anti-mouse IBA1 | N/A | 019-19741 | Immunofluorescence | Wako |
| Donkey anti-rat (H+L) | AlexaFluor 488 | N/A | Immunofluorescence | Invitrogen |
| Donkey anti-rabbit (H+L) | AlexaFluor 647 | N/A | Immunofluorescence | Invitrogen |
List of antibodies, fluorophores, manufacturers, and clones. aAntibody also used for compensation setup