Figure S1.

Cryo-EM Reconstruction, Related to Figure 2

(A) Raw image of SARS-CoV-2 cap(−1) RTC particles in vitreous ice recorded at defocus values from −1.0 to −1.8 μm. Scale bar, 50 nm.

(B) Power spectrum of the image shown in (A), with plot of the rotationally averaged intensity versus resolution. White circle indicates the spatial frequency corresponding to 3.0 Å resolution.

(C) Representative class averages. The edge of each square is ∼367 Å.

(D) Flowchart of SARS-CoV-2 cap(−1) RTC reconstruction. Local resolution estimation is shown at the bottom panel.

(E) Fourier shell correlation (FSC) of the final 3D reconstruction following gold standard refinement. FSC curves are plotted before and after masking.

(F) Angular distribution heatmap of particles used for the refinement.

(G) The 3DFSC sphericity analyzed with 3DFSC in cryoSPARC (Punjani et al., 2017).