. 2020 Nov 11;8:e10172. doi: 10.7717/peerj.10172

Table 1. Tentative compounds identified by ESI-MS in hydroalcoholic extracts from seeds, leaves, and callus of 14 and 38 d of culture from both toxic and non-toxic Jatropha curcas L. varieties.

Compound type/ name	Elemental composition	Mass	Fragment ions in positive ion mode (m/z)	Plant material	Time of culture (d)	Variety	Relative abundance (%)
Phorbol
12-deoxy-16-hydroxy-phorbol	C₂₀H₂₈O₆	364.4	127, 185, 203, 234, 276, 295
				Seeds		T	64.70
						NT	21.05
					Callus	14	T	14.28
						14	NT	8.69
				38		T	30.00
NT	25.00			38
Glycosylated Flavonoids
Apigenin 6-C- α-L-arabinopyranosyl-8-C- β-D-xylopyranoside (m/z 535 [M+H]⁺), and Apigenin 4′-O-rhamnoside (m/z 417 [M+H]⁺)	C₂₅H₂₆O₁₃ and C₂₁H₂₀O₉	534.47 and 416.4	381
				Leaves		T	45.83
						NT	100
					Callus	14	T	100
						14	NT	100
				38		T	100
NT	62.50			38
Vitexin (m/z 433 [M+H]⁺)	C₂₁H₂₀O₁₀	432.37	415
				Leaves		T	27.7
						NT	70.00
					Callus	14	T	10.34
						14	NT	4.76
				38		T	11.11
NT	<12.50			38
Vitexin 4′-O-glucoside-2″-O-rhamnoside (m/z 741 [M+H]⁺)	C₃₃H₄₀O₁₉	740.7	577
				Leaves		T	33.3
						NT	42.84
					Callus	14	T	1.42
						14	NT	6.36
				38		T	12.50
NT	8.75			38
Vicenin-2 (m/z 595 [M+H]⁺)	C₂₇H₃₀O₁₅	594.5	503
				Leaves		T	25.00
						NT	29.16
					Callus	14	T	7.14
						14	NT	4.00
				38		T	6.25
NT	3.75			38
Vicenin-2,6″-O-glucoside (m/z 757 [M+H]⁺)	C₃₃H₄₀O₂₀	756.7	757
				Leaves		T	<9.09
						NT	8.00
					Callus	14	T	1.42
						14	NT	1.73
				38		T	2.22
NT	1.25			38

Notes.