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. 2020 Nov 13;11:5778. doi: 10.1038/s41467-020-19515-0

Fig. 5. Vascular surveillance by platelets prevents pulmonary bleeding.

Fig. 5

a, b LPS-induced (sub-)acute lung injury model. a Representative micrograph of fibrinogen deposition (asterisks) and platelet morphology in the inflamed (LPS) and control lungs (NaCl) of WT animals. Asterisk: lamellipodium. Scale bar = 5 µm. b Exemplary micrograph and quantification of fibrin(ogen) deposition at sites of vascular damage (arrows) compared to control mice indicated by single extravasated erythrocytes and intact vasculature, n = 3 WT mice, paired t test. Scale bar = 5 µm. c, f LPS-induced lung injury in busulfan-treated Arpc2+/+ and Arpc2−/− mice. c Appearance, d hemoglobin content in bronchoalveolar lavage (BAL), and e lung histology 8 h after LPS challenge. Scale bar = 10 mm. f Lung HE stain. Scale bar = 30 µm. d Mann–Whitney. g, h Hyperacute lung injury model. g SpO2 90 min after high-dose tracheal LPS application. Center: mean; whiskers: min–max; box: 25–75th percentiles. h Kaplan–Meier survival curve, n = 8 mice per group. g t Test and h log rank. Error bars = s.e.m. All statistical tests are two-sided. Source data are provided as a Source data file.