Fig. 6. WASp and WAVE play a redundant role in platelet haptotaxis.

a Morphology and migration of WASp−/− platelets compared to controls, n = 3 WASp+/+ and n = 4 WASp−/− mice, t test. Scale bar = 5 µm. b Number of filopodia per cell (Mann–Whitney), solidity (t test), and representative F-actin-stained platelet of Cyfip1−/− (n = 32 cells) and Cyfip1+/+ (n = 30 cells) mice, three mice per group. c, d Representative cell body/filopodia outline and c total cell area of Arpc2−/− (n = 31 cells) and Cyfip1−/− mice (n = 31 cells), and d size of cell body, three mice per group, t test. e p-MLC and F-actin distribution of Arpc2−/− (n = 39 cells) and Cyfip1−/− mice (n = 39 cells), three mice per group. Insets: F-actin distribution between filopodia. Scale bar = 5 µm. e–g Migration of WT, Cyfip1−/−, and Arpc2−/− platelets, e representative micrographs, f percentage of cells migrating and g area cleared per cell, ANOVA and Tukey’s post hoc test, n = 3 mice per group. Scale bar = 5 µm. h Effect of additional Arp2/3 inhibition on Cyfip1−/− platelets. Representative micrographs, and analysis of percentage of cells migrating and area cleared per cell of CK689- and CK666-treated Cyfip1−/− platelets, n = 3 mice per group, paired t test. i Decision-making upon encountering substrate gradients, (left) examples of Cyfip1−/− platelets in contact with interface, and (right) platelet positioning before (0 min) and after migration (45 min), n = 18 cells from three mice. j LPS-induced lung injury in Cyfip1+/+ and Cyfip1−/− mice, quantification of hemoglobin content, erythrocytes, and leukocytes in bronchoalveolar lavage (BAL), n = 3 mice, t test. k LPS-induced lung injury in busulfan-treated, partially platelet-depleted Cyfip1+/+ and Cyfip1−/− mice. Quantification of hemoglobin content, erythrocytes, and leukocytes in bronchoalveolar lavage (BAL), n = 3 mice. Scale bars = 5 µm, t test. Error bars = s.e.m. All statistical tests are two-sided. Source data are provided as a Source data file.