Fig. 2.

a DNA binding motifs enriched for C11orf95^fus1 and C11orf95-RELA^fus1 and C11orf95-RELA¹⁴²⁵ in G16-3, G16-4 and BXD-1425-EPN cells, respectively. b Reporter assay. Reporter EGFP gene was linked to identified C11orf95 binding motif as well as USF1 motif as control. Empty vector (EV), C11orf95-RELA^fus1, RELA, C11orf95^fus1 or activation domain VP64 fused C11orf95^fus1 were expressed to test EGFP expression. EGFP signals were measured by flow cytometry. c C11orf95 binding motif distribution in C11orf95-RELA peaks in G16-4 and BXD-1425-EPN cells. d RELA, C11orf95^fus1, C11orf95-RELA^fus1 and C11orf95-RELA¹⁴²⁵ binding profiles at C11orf95 and RELA genes. The orange bars indicate the regions that have C11orf95 binding motifs