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. 2020 Nov 6;2020:7239895. doi: 10.1155/2020/7239895

Figure 4.

Figure 4

miR-141-3p negatively regulated SIRT1 by direct binding to its 3′-UTR. (a) The predicted binding sites of miR-141-3p and SIRT1. (b) The miR-141-3p mimic and inhibitor was transfected into PC12 cells. Western blot showed that miR-141-3p mimic decreased the SIRT1 protein. The miR-141-3p inhibitor increased the SIRT1 protein. (c) RT-qPCR showed that miR-141-3p mimic decreased SIRT1 mRNA. The miR-141-3p inhibitor increased SIRT1 mRNA. (d) SIRT1 3′-UTR-wild-type or SIRT1 3′-UTR-mutant reporter was transfected along with miR-141-3p mimic into PC12 cells. The luciferase activity was decreased significantly when miR-141-3p mimic was cotransfected with SIRT1 3′-UTR-wild-type reporter. ∗p < 0.05. Error bars indicate standard deviation (SD).