FIGURE 1.

The evaluation of AUF1 in colorectal cancer (CRC) patients and CRC cell lines. A, The expression of AUF1 protein in 20 human CRC tissue (T) and paired adjacent normal tissue (N) specimens by Western blotting. The quantification of the AUF1 protein expression normalized against β‐tubulin was performed using the ImageJ software program. P < .0001. B, The expression of AUF1 protein in human CRC cell lines (SW480, HCT116, LoVo, T84, SW620, COLO320) and the human normal intestinal mucous cell line CCC‐HIE‐2. The quantification of the AUF1 protein expression normalized against β‐tubulin was performed using the ImageJ software program. C, The AUF1 protein expression levels in tissue microarrays, including 87 paired CRC specimens based on the intensity and proportion of immunostaining by Immunohistochemistry. D, Kaplan–Meier curves for overall survival in 87 CRC patients with high or low AUF1 expression levels (P < .001). E. Kaplan–Meier curves for overall survival in 430 CRC patients using TCGA database