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. 2020 Oct 5;9(22):8612–8623. doi: 10.1002/cam4.3464

FIGURE 4.

FIGURE 4

AUF1 regulated colorectal cancer (CRC) through the ERK1/2 and AKT signaling pathways. A, Western blotting to detect the expression of total MEK, phosphorylated MEK, total ERK, phosphorylated ERK, total PDK1, phosphorylated PDK1, total AKT, phosphorylated AKT and CyclinD1 in HCT116 AUF1‐knockdown cells, LoVo AUF1‐knockdown cells and control cells. B. Quantification of the expression of each protein normalized against GAPDH was performed using the Image J software program. C. Western blotting to detect the expression of total MEK, phosphorylated MEK, total ERK, phosphorylated ERK, total PDK1, phosphorylated PDK1, total AKT, phosphorylated AKT, ZEB1 and CyclinD1 in HCT116 AUF1‐rescue cells, LoVo AUF1‐rescue cells, and corresponding AUF1‐knockdown cells. D. In the rescue experiment, quantification of the expression of each protein normalized against GAPDH was performed using the Image J software program