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. 2020 Oct 31;32(5):547–563. doi: 10.21147/j.issn.1000-9604.2020.05.01

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SLC38A3 suppresses transcription of SNAIL by interacting with SETDB1. (A) Western blotting analyses of expression of indicated proteins in siCtrl; (B) Immunofluorescence images of SLC38A3, SETDB1 in KYSE510 cells. Scale bars: 5 µm; (C) Western blotting analyses of immunoprecipitation (IP) products and whole cell lysates derived from KYSE410; (D) Western blotting analyses of expression of total SETDB1 in siCtrl, siSLC38A3_1, siSLC38A3_3 transfected cells derived from KYSE510; (E) Western blotting analyses of expression of indicated proteins in siCtrl, siSLC38A3, siSETDB1 and siSLC38A3+siSETDB1 transfected cells derived from KYSE410; (F) Quantitative RT-PCR analyses of SNAIL levels in siCtrl, siSLC38A3, siSETDB1 and siSLC38A3+siSETDB1 transfected cells derived from KYSE410. SLC38s, solute carrier family 38; IgG, immunoglobulin G; RT-PCR, real-time polymerase chain reaction.