1. Primer sequences.

Gene	Forward Primers (5’→3’)	Reverse Primers (5’→3’)
CCND1, cyclin D1; IL-6, interleukin 6; MMP-1, matrix metalloproteinase-1; Tim-3, T-cell immunoglobulin and mucin-domain containing molecule-3; VEGF, vascular endothelial growth factor; ZO, zona occludens. The Z Sequence is highlighted in bold.
CCND1	CGGTGTCCTACTTCAAATGT	ACTGAACCTGACCGTACAGAAGCGGTCCAGGTAGTTC
C-Myc	TGCTCCATGAGGAGACAC	ACTGAACCTGACCGTACATGATCCAGACTCTGACCTTT
E-cadherin	CACACGGGCTTGGATTT	ACTGAACCTGACCGTACAGACCTCAAAAGGTACCACAT
GAPDH	CTGAGTACGTCGTGGAGTC	ACTGAACCTGACCGTACACAGAGATGATGACCCTTTTG
IL-6	TCATCACTGGTCTTTTGGAG	ACTGAACCTGACCGTACACAGGGGTGGTTATTGCATC
MMP1	CTTTTGTCAGGGGAGATCAT	ACTGAACCTGACCGTACAGGTCCACCTTTCATCTTCAT
NF-κB	ACAGAGAGGATTTCGTTTCC	ACTGAACCTGACCGTACAGTTGCAGATTTTGACCTGAG
Occludin	GAATTCAAACCGAATCATTG	ACTGAACCTGACCGTACATGAAGAATTTCATCTTCTGG
STAT3	CATGGAAGAATCCAACAACG	ACTGAACCTGACCGTACAAATCAGGGAAGCATCACAAT
Tim-3	GCTCCATGTTTTCACATCTT	ACTGAACCTGACCGTACAATTCCACTTCTGAGGACCTT
TWIST	AGCAACAGCGAGGAAGAG	ACTGAACCTGACCGTACAGAGGACCTGGTAGAGGAAGT
VEGFA	GAGCCGGAGAGGGAG	ACTGAACCTGACCGTACACTGGGACCACTTGGCAT
VEGFD	TCCACATTGGAACGATCTGA	ACTGAACCTGACCGTACACTCCACAGCTTCCAGTCCTC
ZO-1	TGACACACATGGTAGACTCA	ACTGAACCTGACCGTACAGTAACTGCGTGAATATTGCT
ZO-2	CAAAAGAGGATTTGGAATTG	ACTGAACCTGACCGTACAGAGCACATCAGAAATGACAA
β-catenin	AAAGGCTACTGTTGGATTGA	ACTGAACCTGACCGTACACTGAACTAGTCGTGGAATGG