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. 2020 Oct;10(5):1245–1255. doi: 10.21037/cdt-20-545

Table 1. Summary of primer sequences used for RT-PCR.

Gene Sequence
HIF1A-AS1 Forward: 5'-AGGATGGAACTATCTGGG-3'
Reverse: 5'-AAGTTAAAGGGAGAAGCTA-3'
TNF-α Forward: 5'-CCCTCACACTCAGATCATCTTCT-3'
Reverse: 5'-GCTACGACGTGGGCTACAG-3'
IL-6 Forward: 5'-TAGTCCTTCCTACCCCAATTTCC-3'
Reverse: 5'-TTGGTCCTTAGCCACTCCTTC-3'
IL-1β Forward: 5'-GCCTCGTGCTGTCGGACC-3'
Reverse: 5'-TGTCGTTGCTTGGTTCTCCTTG-3'
β-actin Forward: 5'-GGCTGTATTCCCCTCCATCG-3'
Reverse: 5'-CCAGTTGGTAACAATGCCATGT-3'