Figure 4.

CCP is a beta-lactamase substrate and can detect low concentrations of beta-lactamase. (a) CCP (0.2 mM) was either mixed with TEM-1 (1 nM) beta-lactamase or PBS at RT and the chemiluminescence at 470 nm was measured over time. TEM-1 produces a 20-fold (relative chemiluminescence intensity at 30 min at 470 nm) higher chemiluminescence signal than CCP mixed with PBS. (b) CCP (0.2 mM) or (c) fluorocilin (0.2 mM) was mixed with various concentrations of TEM-1 beta lactamase at RT for 30 min. CCP has a detection sensitivity of 200 fM. It is 4-orders of magnitude more sensitive than fluorocilin.