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. 2020 Nov 10;14:4801–4813. doi: 10.2147/DDDT.S274812

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© 2020 Wang et al.

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Acacetin suppressed TBHP-induced ROS generation and upregulated the expression and activity of antioxidant proteins. The NPCs were treated with TBHP (50 µM) alone or in combination with acacetin (0.3 µM, 1 µM) for 24 hours. (A, B) Intracellular ROS levels were determined using DCFH-DA with flow cytometry. (C, D) Relative mRNA expression levels of HO-1 and NQO1 were determined by qPCR. (E) Protein expression levels of HO-1 and NQO1 were determined by Western blotting. (F) SOD activities of the NPCs were determined using a SOD assay kit with WST-8. The data are represented as the mean ± SD. n=3. *p<0.05, **p<0.01, ***p<0.001.