FIG 5.

SomA is involved in the cell wall stress response. (A) Summary of the selected cell wall-related genes and the location of their SomA binding motifs. (B) Heat map analysis of the expression of cell wall-related genes. The indicated strains were first cultured in MM with or without doxycycline for 22 h. CFW at a final concentration of 100 μg/ml was then added to the media, and gene expressions were measured at 0.5, 1, and 2 h, respectively. Gene expression was normalized to the endogenous reference gene tubA, and expression is reported relative to the unstressed WT at 22 h growth. All of the results were obtained in three independent biological experiments. (C) Phenotypes of WT and Tet-somA strains cultured on MM or MM supplemented with or without 1 μg/ml doxycycline and with CR, CFW, or CAS. Colony morphology was imaged after 48 h.