FIG 2.
TgMAPK2 forms cytosolic puncta in interphase and early budding parasites. (A) Confocal 0.5-μm slices of TgMAPK23×HA- and GFP–α-tubulin (blue)-expressing parasites were either stained or transiently transfected (Centrin and Rab FP fusions) with the indicated markers. (B) Pearson’s correlation coefficients for TgMAPK23×HA and the indicated markers from panel A (n = ∼20 cells). (C) TgMAPK23×HA intensity diminishes late in the cell cycle. (D) Transcript levels of MAPK2 peak during G1/early S (data from reference 26, retrieved from ToxoDBv43). Bars = 5 μm.