FIG 7.

Prolonged incubation in IAA results in multiple defects in the TgMAPK2^AID parasite ultrastructure. Shown are transmission electron micrographs of intracellular TgMAPK2^AID parasites in the absence of IAA (A and B), with 6 h of growth in IAA (C and D), or with 20 h of growth in IAA (E to H). *, daughter buds; Go, Golgi apparatus; Ap, apicoplast; Mt, mitochondria; RB, residual bodies. Bars = 1 μm. Note that 100% of parasites treated for 20 h with IAA had overduplicated apicoplasts, and 35% ± 5% had overduplicated Golgi apparatus; neither phenotype was observed in untreated parasites.