FIG 8.

Loss of TgMAPK2 does not block organelle replication. (A and B) Z-projections of confocal stacks of TgMAPK2^AID parasites stably expressing mVenus-TgCentrin1 (red) grown for 20 h in the presence or absence of IAA. Parasites were fixed and stained with antibodies for IMC1 (blue), TOM40 (green), or ACP1 (green). Bars = 5 μm. (C to E) The total intensities of TOM40 (mitochondria), ACP (apicoplasts), and IMC1 as well as the areas of the same set of parasites were measured and quantified for each class. AU, arbitrary units. (F) Z-projections of confocal stacks of TgMAPK2^AID parasites stably expressing mVenus-TgCentrin1 (red) and TgGRASP55-mRuby3 (green) grown in the presence or absence of IAA for 8 h and costained with anti-Tgβ-tubulin. Headings in GRASP55 panels indicate Golgi morphological class descriptions. Representative images for each class presenting Golgi morphologies are shown. (G) Quantification of parasites in each class shown in panel F grown in the presence or absence of IAA for 8 h. Means ± SD from 3 biological replicates are shown; 200 to 300 parasites were counted under each condition. P values are from two-tailed Student’s t test.